Hotspot

rps4-trnT-UGU

LSC · 45139-45441 · 303 bp

Back to Dactylorhiza

Pi 0.00748

MarkerSeek score 58.00

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 303
variable_sites: 4
pi: 0.00748
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TCACTTCTGGATCTGGGTCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.4	57.9	450-459 bp	1.0	76.7
rps4-trnT-UGU_p2	TCACTTCTGGATCTGGGTCT	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.4	57.8	418-427 bp	1.0	76.1
rps4-trnT-UGU_p3	TCCAGAGCGTAATTGGGTTT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.1	57.9	475-484 bp	1.0	74.7
rps4-trnT-UGU_p4	TTCCAGAGCGTAATTGGGTT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.1	57.9	476-485 bp	1.0	74.6
rps4-trnT-UGU_p5	TTTCCAGAGCGTAATTGGGT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.1	57.9	477-486 bp	1.0	74.6