Hotspot

trnE-UUC-trnT-GGU

LSC · 30280-31180 · 901 bp

Back to Dactylorhiza

Pi 0.00919

MarkerSeek score 62.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 15
pi: 0.00919
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	948-986 bp	1.0	82.9
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	945-983 bp	1.0	82.8
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	926-964 bp	1.0	82.2
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.7	58.1	923-961 bp	1.0	82.0
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	AACTTACGCCTTACCATGGC	50.0	50.0	57.8	58.3	961-999 bp	1.0	81.4