Hotspot

atpF-atpH

LSC · 12653-12764 · 112 bp

Back to Dactylorhiza

Pi 0.0143

MarkerSeek score 63.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 112
variable_sites: 3
pi: 0.0143
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AACTCGAAACTTCCAACGGA	ATTTATGGACTGGTCGTGGC	45.0	50.0	57.4	58.0	223-231 bp	1.0	78.7
atpF-atpH_p2	AACTCGAAACTTCCAACGGA	GGTCGTGGCATTAGCTCTTT	45.0	50.0	57.4	58.3	212-220 bp	1.0	77.1
atpF-atpH_p3	ACTCGAAACTTCCAACGGAT	ATTTATGGACTGGTCGTGGC	45.0	50.0	57.2	58.0	222-230 bp	1.0	76.9
atpF-atpH_p4	CCACGGAAACAAAAGAATCGG	ATTTATGGACTGGTCGTGGC	47.6	50.0	58.1	58.0	192-200 bp	1.0	75.6
atpF-atpH_p5	ACTCGAAACTTCCAACGGAT	GGTCGTGGCATTAGCTCTTT	45.0	50.0	57.2	58.3	211-219 bp	1.0	75.3