Hotspot

ndhF

SSC · 101549-103768 · 2220 bp

Back to Dactyloctenium

Pi 0.0149
MarkerSeek score 54.00
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2220
variable_sites
33
pi
0.0149
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TTGATGAGCCTCGTTTTGAG AGGTTTGGTGAATCGGAAACA 45.0 42.9 56.1 58.1 2336 bp 1.0 57.2
ndhF_p2 TTTGATGAGCCTCGTTTTGAG AGGTTTGGTGAATCGGAAACA 42.9 42.9 56.8 58.1 2337 bp 1.0 55.2
ndhF_p3 TTGATGAGCCTCGTTTTGAG GGTTTGGTGAATCGGAAACA 45.0 45.0 56.1 56.5 2335 bp 1.0 54.0
ndhF_p4 TTTGATGAGCCTCGTTTTGA AGGTTTGGTGAATCGGAAACA 40.0 42.9 55.6 58.1 2337 bp 1.0 53.4
ndhF_p5 TTTTGATGAGCCTCGTTTTGAG AGGTTTGGTGAATCGGAAACA 40.9 42.9 57.5 58.1 2338 bp 1.0 52.7