Hotspot

ndhE-ndhG

SSC · 108960-109155 · 196 bp

Back to Dactyloctenium

Pi 0.0204

MarkerSeek score 64.20

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 196
variable_sites: 4
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AGCTCTAACCATGTTTCGGC	AGGTGCAATTACTATGGCTCG	50.0	47.6	58.3	58.2	314-319 bp	1.0	69.6
ndhE-ndhG_p2	GCTCTAACCATGTTTCGGCT	AGGTGCAATTACTATGGCTCG	50.0	47.6	58.3	58.2	313-318 bp	1.0	69.6
ndhE-ndhG_p3	AGCTCTAACCATGTTTCGGC	GGTGCAATTACTATGGCTCGA	50.0	47.6	58.3	58.2	313-318 bp	1.0	69.6
ndhE-ndhG_p4	GCTCTAACCATGTTTCGGCT	GGTGCAATTACTATGGCTCGA	50.0	47.6	58.3	58.2	312-317 bp	1.0	69.6
ndhE-ndhG_p5	GCTCTAACCATGTTTCGGCT	GGTGCAATTACTATGGCTCG	50.0	50.0	58.3	56.7	312-317 bp	1.0	68.3