Hotspot

rpl32-trnL-UAG

SSC · 104651-105010 · 360 bp

Back to Dactyloctenium

Pi 0.0279

MarkerSeek score 57.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 360
variable_sites: 10
pi: 0.0279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAGCATCCAAAACCAAAAGGT	TCCTAAGAGCAGCGTGTCTA	42.9	50.0	57.2	57.9	432-599 bp	1.0	66.9
rpl32-trnL-UAG_p2	GAGCATCCAAAACCAAAAGGT	AGCAGCGTGTCTACCAATTT	42.9	45.0	57.2	57.8	425-592 bp	1.0	66.4
rpl32-trnL-UAG_p3	GAGCATCCAAAACCAAAAGGT	GCTTGAGCACTGCTTCCTAA	42.9	50.0	57.2	58.2	446-613 bp	1.0	66.3
rpl32-trnL-UAG_p4	GAGCATCCAAAACCAAAAGGT	CCTAAGAGCAGCGTGTCTAC	42.9	55.0	57.2	57.8	431-598 bp	1.0	66.3
rpl32-trnL-UAG_p5	GAGCATCCAAAACCAAAAGGT	CTAAGAGCAGCGTGTCTACC	42.9	55.0	57.2	57.8	430-597 bp	1.0	66.3