Hotspot

rpl14-rpl16

LSC · 77586-77696 · 111 bp

Back to Dactyloctenium

Pi 0.0521

MarkerSeek score 71.50

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 111
variable_sites: 5
pi: 0.0521
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CTAGCTCCACTGTTATCCGC	AATGCCCATACGAAGCCAAT	55.0	45.0	57.9	57.9	186-201 bp	1.0	84.4
rpl14-rpl16_p2	CTAGCTCCACTGTTATCCGC	ATGCCCATACGAAGCCAATT	55.0	45.0	57.9	57.9	185-200 bp	1.0	84.4
rpl14-rpl16_p3	GCTCCACTGTTATCCGCTAC	AATGCCCATACGAAGCCAAT	55.0	45.0	58.1	57.9	183-198 bp	1.0	84.4
rpl14-rpl16_p4	GCTCCACTGTTATCCGCTAC	ATGCCCATACGAAGCCAATT	55.0	45.0	58.1	57.9	182-197 bp	1.0	84.4
rpl14-rpl16_p5	AGCTCCACTGTTATCCGCTA	ATGCCCATACGAAGCCAATT	50.0	45.0	58.2	57.9	183-198 bp	1.0	83.7