Hotspot

clpP-psbB

LSC · 68347-68858 · 512 bp

Back to Dactyloctenium

Pi 0.016

MarkerSeek score 66.30

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 512
variable_sites: 8
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACCCAAGTCGCTTCTTCATC	TATGCACCGAAAGCAATCGA	50.0	45.0	57.9	58.0	633-644 bp	1.0	81.4
clpP-psbB_p2	TCAACCCAAGTCGCTTCTTC	TATGCACCGAAAGCAATCGA	50.0	45.0	58.1	58.0	636-647 bp	1.0	81.3
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	TATGCACCGAAAGCAATCGA	50.0	45.0	57.8	58.0	593-604 bp	1.0	81.1
clpP-psbB_p4	ACCCAAGTCGCTTCTTCATC	CCAGCCCAACCAGAAACTAG	50.0	55.0	57.9	58.2	667-678 bp	1.0	80.1
clpP-psbB_p5	ACCCAAGTCGCTTCTTCATC	TATAAAGCCATCGAGCCAGC	50.0	50.0	57.9	57.8	682-693 bp	1.0	80.0