Hotspot

ndhC-trnV-UAC

LSC · 50344-51278 · 935 bp

Back to Dactyloctenium

Pi 0.0173

MarkerSeek score 69.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 935
variable_sites: 16
pi: 0.0173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCTGGTCCTTCACTACTCGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.2	57.9	1068-1073 bp	1.0	80.0
ndhC-trnV-UAC_p2	TCTGGTCCTTCACTACTCGG	AGCCCAATGCAGAAAAATGC	55.0	45.0	58.2	57.9	1161-1166 bp	1.0	79.6
ndhC-trnV-UAC_p3	CACTACTCGGGGCTAAAAGC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.3	57.9	1058-1063 bp	1.0	78.6
ndhC-trnV-UAC_p4	CACTACTCGGGGCTAAAAGC	AGCCCAATGCAGAAAAATGC	55.0	45.0	58.3	57.9	1151-1156 bp	1.0	78.2
ndhC-trnV-UAC_p5	TCTGGTCCTTCACTACTCGG	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.2	58.3	1069-1074 bp	1.0	78.2