Hotspot

petN-trnC-GCA

LSC · 17676-18472 · 797 bp

Back to Dactyloctenium

Pi 0.0227

MarkerSeek score 61.70

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 18
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	846-900 bp	1.0	72.4
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	848-902 bp	1.0	71.9
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	CAGTTCAAATCTGGGTGCCG	50.0	55.0	58.2	59.5	847-901 bp	1.0	69.0
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	AGGGGACTGCAAATCCTTTA	50.0	45.0	58.2	56.4	871-925 bp	1.0	68.0
petN-trnC-GCA_p5	AGCCCAAGCGAGACTTACTA	TGCAAATCCTTTATCCCCAGT	50.0	42.9	58.2	57.2	864-918 bp	1.0	66.7