Hotspot

trnY-GUA-trnD-GUC

LSC · 15633-15975 · 343 bp

Back to Dactyloctenium

Pi 0.0175

MarkerSeek score 63.50

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 343
variable_sites: 6
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	AATTCTTCCTGGGTCGATGC	GCGGTGCTCTAACCAATTGA	50.0	50.0	58.0	58.6	468-470 bp	1.0	76.3
trnY-GUA-trnD-GUC_p2	AATTCTTCCTGGGTCGATGC	ACTCTAGTTCGGGACTGACG	50.0	55.0	58.0	58.5	520-522 bp	1.0	76.2
trnY-GUA-trnD-GUC_p3	GGGGACGGACTGTAAATTCG	GCGGTGCTCTAACCAATTGA	55.0	50.0	58.4	58.6	435-437 bp	1.0	73.9
trnY-GUA-trnD-GUC_p4	GGGGACGGACTGTAAATTCG	ACTCTAGTTCGGGACTGACG	55.0	55.0	58.4	58.5	487-489 bp	1.0	73.8
trnY-GUA-trnD-GUC_p5	GGGACGGACTGTAAATTCGT	ACTCTAGTTCGGGACTGACG	50.0	55.0	57.6	58.5	486-488 bp	1.0	73.6