Hotspot

trnT-GGU-trnE-UUC

LSC · 14909-15414 · 506 bp

Back to Dactyloctenium

Pi 0.0238
MarkerSeek score 56.50
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
506
variable_sites
12
pi
0.0238
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 GTGGTAGAGTAATGCCACGG GCATCGACCCAGGAAGAATT 55.0 50.0 58.1 58.0 704-705 bp 1.0 81.0
trnT-GGU-trnE-UUC_p2 GACCAAAGTCATCGGTTCGA GCATCGACCCAGGAAGAATT 50.0 50.0 57.9 58.0 681-682 bp 1.0 80.9
trnT-GGU-trnE-UUC_p3 AGACCAAAGTCATCGGTTCG GCATCGACCCAGGAAGAATT 50.0 50.0 57.9 58.0 682-683 bp 1.0 80.9
trnT-GGU-trnE-UUC_p4 GTGGTAGAGTAATGCCACGG TTGAACTACAATCCCTGCGG 55.0 50.0 58.1 57.9 1136-1139 bp 1.0 80.2
trnT-GGU-trnE-UUC_p5 CCCGTTGAAAATTGCTGCAA GCATCGACCCAGGAAGAATT 45.0 50.0 58.1 58.0 1288-1338 bp 1.0 80.0