Hotspot

psbC-trnS-UGA

LSC · 11564-11719 · 156 bp

Back to Dactyloctenium

Pi 0.0323

MarkerSeek score 68.30

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 5
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CATTTGTGGCATGCAGGAAG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	344-345 bp	1.0	81.2
psbC-trnS-UGA_p2	AGCAGGCTTTGAAAAGGGAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	57.9	58.0	309-310 bp	1.0	80.7
psbC-trnS-UGA_p3	ATTTGTGGCATGCAGGAAGA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.1	58.0	343-344 bp	1.0	80.6
psbC-trnS-UGA_p4	CATTTGTGGCATGCAGGAAG	ATAGCCCCGGTCTTGAAAAC	50.0	50.0	58.0	57.9	324-325 bp	1.0	80.4
psbC-trnS-UGA_p5	ATTTGTGGCATGCAGGAAGA	ATAGCCCCGGTCTTGAAAAC	45.0	50.0	58.1	57.9	323-324 bp	1.0	79.9