Hotspot

rps16-trnQ-UUG

LSC · 5598-6864 · 1267 bp

Back to Dactyloctenium

Pi 0.0183

MarkerSeek score 66.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1267
variable_sites: 22
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1291-1341 bp	1.0	83.4
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	CGCATAAAAGAAATGGGGCG	45.0	50.0	58.1	58.2	1352-1402 bp	1.0	82.5
rps16-trnQ-UUG_p3	TCATCTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1302-1352 bp	1.0	81.6
rps16-trnQ-UUG_p4	TCATCTCCTTCAAGTCGCAC	CGCATAAAAGAAATGGGGCG	50.0	50.0	57.6	58.2	1363-1413 bp	1.0	80.6
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1295-1345 bp	1.0	78.7