Hotspot

psbB

LSC · 67953-69476 · 1524 bp

Back to Dactylis

Pi 0.00131

MarkerSeek score 59.50

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 2
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	TGGATAGTCTTTTCCAATGCGA	GGGAGCTGTTTTGTTTCCCA	40.9	50.0	57.8	58.6	1661 bp	1.0	57.6
psbB_p2	TGGATAGTCTTTTCCAATGCGA	TGGGAGCTGTTTTGTTTCCC	40.9	50.0	57.8	58.6	1662 bp	1.0	57.6
psbB_p3	TGGATAGTCTTTTCCAATGCGA	AAGGATGGGAGCTGTTTTGT	40.9	45.0	57.8	57.3	1667 bp	1.0	56.9
psbB_p4	TGGATAGTCTTTTCCAATGCGA	AGGATGGGAGCTGTTTTGTT	40.9	45.0	57.8	57.3	1666 bp	1.0	56.9
psbB_p5	TGGATAGTCTTTTCCAATGCGA	GGAGCTGTTTTGTTTCCCATG	40.9	47.6	57.8	58.0	1660 bp	1.0	54.1