Hotspot

petA

LSC · 58745-59707 · 963 bp

Back to Dactylis

Pi 0

MarkerSeek score 40.00

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	GAAATTCAGGGATCCGCGAT	TGAGGCTTTTTACCAACTTGG	50.0	42.9	58.1	56.6	1028 bp	1.0	60.1
petA_p2	GAAATTCAGGGATCCGCGAT	GCAATTGCCAATAAATTGAGGC	50.0	40.9	58.1	57.5	1044 bp	1.0	58.7
petA_p3	AAATTCAGGGATCCGCGATT	TGAGGCTTTTTACCAACTTGG	45.0	42.9	57.7	56.6	1027 bp	1.0	58.2
petA_p4	AATTCAGGGATCCGCGATTG	TGAGGCTTTTTACCAACTTGG	50.0	42.9	58.4	56.6	1026 bp	1.0	57.8
petA_p5	GAAATTCAGGGATCCGCGAT	TTGAGGCTTTTTACCAACTTGG	50.0	40.9	58.1	57.3	1029 bp	1.0	57.8