Hotspot

clpP-psbB

LSC · 67433-67952 · 520 bp

Back to Dactylis

Pi 0.00193

MarkerSeek score 49.20

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 520
variable_sites: 1
pi: 0.00193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACCCAAGTCGCTTCTTCATC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	601-602 bp	1.0	77.6
clpP-psbB_p2	TCAACCCAAGTCGCTTCTTC	CGATACCAAGGCAAACCCAT	50.0	50.0	58.1	58.2	604-605 bp	1.0	77.4
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	561-562 bp	1.0	77.0
clpP-psbB_p4	TTTGGAACACCAATGGGCAT	CGATACCAAGGCAAACCCAT	45.0	50.0	58.3	58.2	559-560 bp	1.0	76.2
clpP-psbB_p5	AAGTCGCTTCTTCATCTCCG	CGATACCAAGGCAAACCCAT	50.0	50.0	57.7	58.2	597-598 bp	1.0	76.1