Hotspot

petN-trnC-GCA

LSC · 16927-17865 · 939 bp

Back to Dactylis

Pi 0.00319

MarkerSeek score 60.60

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 939
variable_sites: 3
pi: 0.00319
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	TTTCTCGATTTTCGCTCGGA	50.0	45.0	58.2	57.6	1063-1064 bp	1.0	76.2
petN-trnC-GCA_p2	CCACTCCTCCCCCATACTAC	TTTCTCGATTTTCGCTCGGA	60.0	45.0	58.3	57.6	1119-1120 bp	1.0	75.1
petN-trnC-GCA_p3	TCCACTCCTCCCCCATACTA	TTTCTCGATTTTCGCTCGGA	55.0	45.0	58.4	57.6	1120-1121 bp	1.0	74.4
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	TCTCGATTTTCGCTCGGATT	50.0	45.0	58.2	57.4	1061-1062 bp	1.0	74.4
petN-trnC-GCA_p5	AGCCCAAGCGAGACTTACTA	TTCTCGATTTTCGCTCGGAT	50.0	45.0	58.2	57.4	1062-1063 bp	1.0	74.4