Hotspot

trnG-UCC-trnT-GGU

LSC · 13616-14543 · 928 bp

Back to Dactylis

Pi 0.00216

MarkerSeek score 60.00

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 2
pi: 0.00216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	CCGAGGACTTACGTCTTAGC	38.1	55.0	55.5	57.5	1082 bp	1.0	48.1
trnG-UCC-trnT-GGU_p2	AGAACGAATCACACTTTTACCAC	CCGAGGACTTACGTCTTAGC	39.1	55.0	57.3	57.5	1085 bp	1.0	47.0
trnG-UCC-trnT-GGU_p3	GAACGAATCACACTTTTACCACT	CCGAGGACTTACGTCTTAGC	39.1	55.0	57.3	57.5	1084 bp	1.0	47.0
trnG-UCC-trnT-GGU_p4	AACGAATCACACTTTTACCACT	CCGAGGACTTACGTCTTAGC	36.4	55.0	56.2	57.5	1083 bp	1.0	46.2
trnG-UCC-trnT-GGU_p5	ACGAATCACACTTTTACCACT	GGACTTACGTCTTAGCACGA	38.1	50.0	55.5	57.1	1078 bp	1.0	44.6