Hotspot

trnK-UUU-rps16

LSC · 3955-4513 · 559 bp

Back to Dactylis

Pi 0.00358

MarkerSeek score 45.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 559
variable_sites: 2
pi: 0.00358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TGGACGAATTTGTTGCTCCA	TCGAGCCGTATGAGGAGAAA	45.0	50.0	58.0	58.2	935-943 bp	1.0	78.1
trnK-UUU-rps16_p2	TGGACGAATTTGTTGCTCCA	TTTCTCGAGCCGTATGAGGA	45.0	50.0	58.0	58.2	939-947 bp	1.0	78.1
trnK-UUU-rps16_p3	TGAGGATCAGTCGTGGTCTT	TCGAGCCGTATGAGGAGAAA	50.0	50.0	58.1	58.2	974-982 bp	1.0	77.7
trnK-UUU-rps16_p4	TGAGGATCAGTCGTGGTCTT	TTTCTCGAGCCGTATGAGGA	50.0	50.0	58.1	58.2	978-986 bp	1.0	77.7
trnK-UUU-rps16_p5	TTTTTGCAGGCCAATTGCTC	TCGAGCCGTATGAGGAGAAA	45.0	50.0	58.1	58.2	1369-1377 bp	1.0	77.5