Hotspot

psbC

LSC · 35851-37272 · 1422 bp

Back to Dactyladenia

Pi 0.00188

MarkerSeek score 59.90

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 4
pi: 0.00188
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTCTCAGGAAATCCGTGCAG	ACTTGTAGAATCCAATTGACTCCT	50.0	37.5	57.9	57.6	1584 bp	1.0	44.4
psbC_p2	GCAGCGGAAGATCCTGAATT	ACTTGTAGAATCCAATTGACTCCT	50.0	37.5	58.3	57.6	1568 bp	1.0	42.5
psbC_p3	AGGGTATTCGTGCTTGGATG	ACTTGTAGAATCCAATTGACTCCT	50.0	37.5	57.7	57.6	1513 bp	1.0	42.5
psbC_p4	TTCTCAGGAAATCCGTGCAG	AGAATCCAATTGACTCCTACTTCA	50.0	37.5	57.9	57.4	1578 bp	1.0	42.4
psbC_p5	TTCTCAGGAAATCCGTGCAG	TCCAATTGACTCCTACTTCA	50.0	40.0	57.9	53.3	1574 bp	1.0	41.6