Hotspot

clpP

LSC · 74172-76243 · 2072 bp

Back to Dactyladenia

Pi 0.00322

MarkerSeek score 55.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2072
variable_sites: 10
pi: 0.00322
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GCGATTGCATAAAATTCCTGC	ACGCTTTCACATCAAAGTGA	42.9	40.0	56.9	55.9	2150-2152 bp	1.0	47.7
clpP_p2	GCGATTGCATAAAATTCCTGCT	ACGCTTTCACATCAAAGTGA	40.9	40.0	58.3	55.9	2150-2152 bp	1.0	46.3
clpP_p3	GCGATTGCATAAAATTCCTGC	ACGCTTTCACATCAAAGTGAT	42.9	38.1	56.9	56.5	2150-2152 bp	1.0	44.0
clpP_p4	GCGATTGCATAAAATTCCTGCT	ACGCTTTCACATCAAAGTGAT	40.9	38.1	58.3	56.5	2150-2152 bp	1.0	42.7
clpP_p5	GCGATTGCATAAAATTCCTGC	GTTACGCTTTCACATCAAAGTGA	42.9	39.1	56.9	57.9	2153-2155 bp	1.0	41.3