Hotspot

cemA

LSC · 64591-65310 · 720 bp

Back to Dactyladenia

Pi 0.00186

MarkerSeek score 60.30

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 720
variable_sites: 2
pi: 0.00186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	GCGGAATGGGTTCAGAGATT	AGAATCCGAAACTCTTAGATGGA	50.0	39.1	58.0	56.8	900-903 bp	1.0	46.5
cemA_p2	GCGGAATGGGTTCAGAGATT	TGAGAAGAATCCGAAACTCT	50.0	40.0	58.0	53.7	905-908 bp	1.0	45.8
cemA_p3	GCGGAATGGGTTCAGAGATT	TGAGAAGAATCCGAAACTCTT	50.0	38.1	58.0	54.6	905-908 bp	1.0	44.9
cemA_p4	GCGGAATGGGTTCAGAGATT	AAGAATCCGAAACTCTTAGATGGA	50.0	37.5	58.0	57.5	901-904 bp	1.0	43.7
cemA_p5	GCGGAATGGGTTCAGAGATT	AGAATCCGAAACTCTTAGATGGAA	50.0	37.5	58.0	57.5	900-903 bp	1.0	43.7