Hotspot

petA-psbJ

LSC · 66490-67289 · 800 bp

Back to Dactyladenia

Pi 0.00667

MarkerSeek score 58.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 800
variable_sites: 8
pi: 0.00667
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GACCCATTACGTGTTCAAGGA	GGGTCATATTCCGGATTGGG	47.6	55.0	58.0	57.8	953-954 bp	1.0	71.1
petA-psbJ_p2	CCCATTACGTGTTCAAGGACT	GGGTCATATTCCGGATTGGG	47.6	55.0	57.9	57.8	951-952 bp	1.0	71.1
petA-psbJ_p3	ACCCATTACGTGTTCAAGGAC	GGGTCATATTCCGGATTGGG	47.6	55.0	58.2	57.8	952-953 bp	1.0	69.7
petA-psbJ_p4	ACCCATTACGTGTTCAAGGA	GGGTCATATTCCGGATTGGG	45.0	55.0	56.8	57.8	952-953 bp	1.0	69.6
petA-psbJ_p5	GACCCATTACGTGTTCAAGGA	GGTCATATTCCGGATTGGGC	47.6	55.0	58.0	58.5	952-953 bp	1.0	69.0