Hotspot

trnF-GAA-ndhJ

LSC · 50433-51224 · 792 bp

Back to Dactyladenia

Pi 0.00589

MarkerSeek score 37.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 7
pi: 0.00589
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TCAACCGACGTATGCTTCTG	55.0	50.0	58.0	58.0	1544-1550 bp	1.0	80.7
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	GACGTATGCTTCTGCCTCAA	55.0	50.0	58.0	58.0	1538-1544 bp	1.0	80.7
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	AAATGCAGGGTCGTTTGTCT	55.0	45.0	58.0	58.0	1335-1341 bp	1.0	80.7
trnF-GAA-ndhJ_p4	GCATCAGAAATGGTCGGGAT	TCAACCGACGTATGCTTCTG	50.0	50.0	58.0	58.0	1559-1565 bp	1.0	80.5
trnF-GAA-ndhJ_p5	GCATCAGAAATGGTCGGGAT	GACGTATGCTTCTGCCTCAA	50.0	50.0	58.0	58.0	1553-1559 bp	1.0	80.5