Hotspot

trnE-UUC-trnT-GGU

LSC · 32479-33157 · 679 bp

Back to Dactyladenia

Pi 0.00393

MarkerSeek score 66.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 4
pi: 0.00393
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	755-782 bp	1.0	81.4
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	734-761 bp	1.0	81.3
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	734-761 bp	1.0	81.0
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	713-740 bp	1.0	81.0
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.3	58.1	731-758 bp	1.0	80.8