Hotspot

trnC-GCA-petN

LSC · 28466-29448 · 983 bp

Back to Dactyladenia

Pi 0.00543

MarkerSeek score 47.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 983
variable_sites: 8
pi: 0.00543
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGTGTCGCCTGATTAACAA	AGTCCACCTCTTCCCCATAC	50.0	55.0	58.2	58.1	1083-1090 bp	1.0	78.7
trnC-GCA-petN_p2	GGGTGTCGCCTGATTAACAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.2	58.2	1024-1031 bp	1.0	78.5
trnC-GCA-petN_p3	GGGTGTCGCCTGATTAACAA	GAGTCCACCTCTTCCCCATA	50.0	55.0	58.2	57.8	1084-1091 bp	1.0	78.2
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGTCCACCTCTTCCCCATAC	50.0	55.0	58.4	58.1	1119-1126 bp	1.0	77.3
trnC-GCA-petN_p5	CTGCAAATCCTTTTTCCCCG	AGTCCACCTCTTCCCCATAC	50.0	55.0	57.6	58.1	1113-1120 bp	1.0	77.2