Hotspot

rpoB-trnC-GCA

LSC · 27078-28384 · 1307 bp

Back to Dactyladenia

Pi 0.00204
MarkerSeek score 35.20
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1307
variable_sites
4
pi
0.00204
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AAGACTAGATCCCCGCCTAC ACAAAGTATCTTCCGCGCTT 55.0 45.0 58.0 57.9 2389-2418 bp 1.0 80.6
rpoB-trnC-GCA_p2 TACCCTTCCTCCCCAATCTG ACAAAGTATCTTCCGCGCTT 55.0 45.0 58.1 57.9 2147-2176 bp 1.0 79.9
rpoB-trnC-GCA_p3 TGGCATTTTCCTTTGACCCA ACAAAGTATCTTCCGCGCTT 45.0 45.0 57.9 57.9 2340-2369 bp 1.0 79.8
rpoB-trnC-GCA_p4 AAGACTAGATCCCCGCCTAC TTGTTAATCAGGCGACACCC 55.0 50.0 58.0 58.2 2081-2103 bp 1.0 79.6
rpoB-trnC-GCA_p5 AAAAGACTAGATCCCCGCCT ACAAAGTATCTTCCGCGCTT 50.0 45.0 58.1 57.9 2391-2420 bp 1.0 79.6