Hotspot

rpoA

LSC · 5467-6462 · 996 bp

Back to Cytisus

Pi 0.01

MarkerSeek score 50.50

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 996
variable_sites: 15
pi: 0.01
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CGGGCGGAAGTCATGATAAA	TAATGGTTTCGGTACCTGCG	50.0	50.0	58.1	58.0	1622-1628 bp	1.0	82.1
rpoA_p2	CGGGCGGAAGTCATGATAAA	GGTTGGGTATTGGAGCAACA	50.0	50.0	58.1	58.1	1496-1502 bp	1.0	81.6
rpoA_p3	CGGGCGGAAGTCATGATAAA	GTGGTTGGGTATTGGAGCAA	50.0	50.0	58.1	58.1	1498-1504 bp	1.0	81.6
rpoA_p4	CGGGCGGAAGTCATGATAAA	TGGTTGGGTATTGGAGCAAC	50.0	50.0	58.1	58.1	1497-1503 bp	1.0	81.6
rpoA_p5	TGGGTTCGCGTAAAAATGGA	TAATGGTTTCGGTACCTGCG	45.0	50.0	58.1	58.0	1867-1873 bp	1.0	81.4