Hotspot

ndhI-ndhG

SSC · 118299-118650 · 352 bp

Back to Cytisus

Pi 0.0421

MarkerSeek score 70.90

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 352
variable_sites: 21
pi: 0.0421
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	CAAACAATCCGAGCTGCAAG	GTGCTGCAGCTACAAAATGG	50.0	50.0	58.0	58.0	994-1013 bp	1.0	83.4
ndhI-ndhG_p2	CAAACAATCCGAGCTGCAAG	AGCCACAACAATTGCACCTA	50.0	45.0	58.0	58.0	1332-1351 bp	1.0	82.7
ndhI-ndhG_p3	ACGGTCAACAAACAATCCGA	AGCCACAACAATTGCACCTA	45.0	45.0	58.0	58.0	1340-1359 bp	1.0	82.6
ndhI-ndhG_p4	CACAACAAGATGGACGTTGC	GTGCTGCAGCTACAAAATGG	50.0	50.0	58.0	58.0	1231-1250 bp	1.0	82.6
ndhI-ndhG_p5	CACAACAAGATGGACGTTGC	AGCCACAACAATTGCACCTA	50.0	45.0	58.0	58.0	1569-1588 bp	1.0	82.2