Hotspot

ycf1-rps15

SSC · 113488-113911 · 424 bp

Back to Cytisus

Pi 0.0339

MarkerSeek score 66.30

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 424
variable_sites: 19
pi: 0.0339
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	ATTGAGCTGGAGAGGTTCCT	ACAGTAGTCGTTGCCGTTTT	50.0	45.0	58.0	58.1	1365-1371 bp	1.0	82.6
ycf1-rps15_p2	ATTGAGCTGGAGAGGTTCCT	CCAACGTGTTACATAGGGCA	50.0	50.0	58.0	57.9	1756-1777 bp	1.0	82.5
ycf1-rps15_p3	ATTGAGCTGGAGAGGTTCCT	GACTCCCGAATAGCCAACTG	50.0	55.0	58.0	58.1	1432-1438 bp	1.0	82.4
ycf1-rps15_p4	ATTGAGCTGGAGAGGTTCCT	TATTTCCCAACTGTTCCGGC	50.0	50.0	58.0	58.2	1820-1841 bp	1.0	81.8
ycf1-rps15_p5	ATTGAGCTGGAGAGGTTCCT	TAAGTCGAAGAACACCGTGC	50.0	50.0	58.0	58.2	1631-1652 bp	1.0	81.8