Hotspot

trnH-rpl2

LSC · 82695-82803 · 109 bp

Back to Cytisus

Pi 0.0412

MarkerSeek score 70.50

Primer available yes

Label trnH-rpl2

Feature Metadata

label_name: trnH-rpl2
feature_type: igs
region: LSC
length_bp: 109
variable_sites: 6
pi: 0.0412
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-rpl2_p1	TGGATCAAGGCAGTGGATTG	CTGCGTCGCCGTAGTAAATA	50.0	50.0	57.9	57.9	177-189 bp	1.0	84.7
trnH-rpl2_p2	TGGATCAAGGCAGTGGATTG	TGCGTCGCCGTAGTAAATAG	50.0	50.0	57.9	57.9	176-188 bp	1.0	84.7
trnH-rpl2_p3	GGATCAAGGCAGTGGATTGT	TGCGTCGCCGTAGTAAATAG	50.0	50.0	57.9	57.9	175-187 bp	1.0	84.7
trnH-rpl2_p4	CGGATGTAGCCAAGTGGATC	CTGCGTCGCCGTAGTAAATA	55.0	50.0	58.1	57.9	191-203 bp	1.0	84.5
trnH-rpl2_p5	CGGATGTAGCCAAGTGGATC	TGCGTCGCCGTAGTAAATAG	55.0	50.0	58.1	57.9	190-202 bp	1.0	84.5