Hotspot

psbA-trnH

LSC · 82349-82619 · 271 bp

Back to Cytisus

Pi 0.0531

MarkerSeek score 75.00

Primer available yes

Label psbA-trnH

Feature Metadata

label_name: psbA-trnH
feature_type: igs
region: LSC
length_bp: 271
variable_sites: 20
pi: 0.0531
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnH_p1	GGTATCTGGTTTACCGCGTT	GGTTATCCTGCCCTTGGAAG	50.0	55.0	58.0	57.9	753-755 bp	1.0	86.1
psbA-trnH_p2	GGTATCTGGTTTACCGCGTT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	791-793 bp	1.0	85.8
psbA-trnH_p3	GGTATCTGGTTTACCGCGTT	GAGAAGTTTGGGTAGAGCCG	50.0	55.0	58.0	58.0	898-900 bp	1.0	85.5
psbA-trnH_p4	ATCCGATTGGTCAAGGAAGC	GAGAAGTTTGGGTAGAGCCG	50.0	55.0	58.0	58.0	1260-1262 bp	1.0	83.8
psbA-trnH_p5	TCAGCACCATGGCTTTCAAT	GAGAAGTTTGGGTAGAGCCG	45.0	55.0	58.1	58.0	873-875 bp	0.666667	69.1