Hotspot

trnM-atpE

LSC · 73272-73405 · 134 bp

Back to Cytisus

Pi 0.0697

MarkerSeek score 75.50

Primer available yes

Label trnM-atpE

Feature Metadata

label_name: trnM-atpE
feature_type: igs
region: LSC
length_bp: 134
variable_sites: 14
pi: 0.0697
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-atpE_p1	TGGTTCAAATCCAATAGTAGGT	TCTCAGACGAGCTAGGACAC	36.4	55.0	54.8	58.3	206-212 bp	1.0	45.8
trnM-atpE_p2	TGGTTCAAATCCAATAGTAGGT	CTCTCAGACGAGCTAGGACA	36.4	55.0	54.8	58.0	207-213 bp	1.0	45.7
trnM-atpE_p3	TTGGTTCAAATCCAATAGTAGGT	CTCTCAGACGAGCTAGGACA	34.8	55.0	55.6	58.0	208-214 bp	1.0	45.7
trnM-atpE_p4	AGTCATTGGTTCAAATCCAA	CTCTCAGACGAGCTAGGACA	35.0	55.0	52.9	58.0	213-219 bp	1.0	45.6
trnM-atpE_p5	AGTCATTGGTTCAAATCCAA	TCTCAGACGAGCTAGGACAC	35.0	55.0	52.9	58.3	212-218 bp	1.0	45.6