Hotspot

psbM-petN

LSC · 45398-46545 · 1148 bp

Back to Cytisus

Pi 0.0215

MarkerSeek score 64.30

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 1148
variable_sites: 37
pi: 0.0215
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AGTTCCGACTGCCTTTTTACT	TCTTGCTTGGGCTGCTTTAA	42.9	45.0	57.5	58.0	1282-1301 bp	1.0	70.5
psbM-petN_p2	TCATTTTAGTTCCGACTGCCT	TCTTGCTTGGGCTGCTTTAA	42.9	45.0	57.3	58.0	1289-1308 bp	1.0	68.9
psbM-petN_p3	AGTTCCGACTGCCTTTTTACT	ATGGGGAAGAAGTGGACTCT	42.9	50.0	57.5	57.6	1228-1247 bp	1.0	67.9
psbM-petN_p4	AGTTCCGACTGCCTTTTTACT	CCCTCGTAGTATGGGGAAGA	42.9	55.0	57.5	57.6	1238-1257 bp	1.0	67.7
psbM-petN_p5	AGTTCCGACTGCCTTTTTACT	TCCCTCGTAGTATGGGGAAG	42.9	55.0	57.5	57.6	1239-1258 bp	1.0	67.7