Hotspot

cemA-ycf4

LSC · 23817-24158 · 342 bp

Back to Cytisus

Pi 0.0343

MarkerSeek score 68.00

Primer available yes

Label cemA-ycf4

Feature Metadata

label_name: cemA-ycf4
feature_type: igs
region: LSC
length_bp: 342
variable_sites: 17
pi: 0.0343
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-ycf4_p1	ATGAAAGAGATGCACCAGGG	TATTTCTTGCGCGTCCCAAT	50.0	45.0	57.6	58.3	445-452 bp	1.0	80.4
cemA-ycf4_p2	ATGAAAGAGATGCACCAGGG	CTATTTCTTGCGCGTCCCAA	50.0	50.0	57.6	58.9	446-453 bp	1.0	75.1
cemA-ycf4_p3	ATGAAAGAGATGCACCAGGG	GCGCGTCCCAATTGAAGTAT	50.0	50.0	57.6	59.0	437-444 bp	1.0	74.6
cemA-ycf4_p4	ATGAAAGAGATGCACCAGGG	ATTTCTTGCGCGTCCCAATT	50.0	45.0	57.6	59.1	444-451 bp	1.0	73.5
cemA-ycf4_p5	ATGAAAGAGATGCACCAGGG	TATTTCTTGCGCGTCCCAA	50.0	47.4	57.6	57.8	445-452 bp	1.0	72.5