Hotspot

rpl16-rpl14

LSC · 2906-3038 · 133 bp

Back to Cytisus

Pi 0.0365

MarkerSeek score 66.80

Primer available yes

Label rpl16-rpl14

Feature Metadata

label_name: rpl16-rpl14
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 7
pi: 0.0365
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rpl14_p1	ACTCAATTCATTATTTCGGGA	CCGCTGTTATCGGCTACATT	33.3	50.0	52.9	58.1	188-201 bp	1.0	46.9
rpl16-rpl14_p2	AACTCAATTCATTATTTCGGGA	CCGCTGTTATCGGCTACATT	31.8	50.0	53.8	58.1	189-202 bp	1.0	46.9
rpl16-rpl14_p3	ACTCAATTCATTATTTCGGGAT	CCGCTGTTATCGGCTACATT	31.8	50.0	53.6	58.1	188-201 bp	1.0	46.9
rpl16-rpl14_p4	GAACTCAATTCATTATTTCGGGA	CCGCTGTTATCGGCTACATT	34.8	50.0	55.0	58.1	190-203 bp	1.0	46.8
rpl16-rpl14_p5	GAACTCAATTCATTATTTCGGG	CCGCTGTTATCGGCTACATT	36.4	50.0	53.6	58.1	190-203 bp	1.0	46.8