Hotspot

ycf1

SSC · 119831-124939 · 5109 bp

Back to Cyrtomium

Pi 0.0109

MarkerSeek score 39.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5109
variable_sites: 124
pi: 0.0109
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCAGTTCTCGTCACTTCCAG	ATTGCTTCGCCAAAATTCCG	55.0	45.0	57.9	58.0	1243-1246 bp	1.0	81.8
ycf1_p2	CCAGTTCTCGTCACTTCCAG	TGTAGCGGCTAGATCAGACA	55.0	50.0	57.9	57.9	1369-1372 bp	1.0	81.2
ycf1_p3	CCAGTTCTCGTCACTTCCAG	GTGAGGAGTTTTCGCAGGAT	55.0	50.0	57.9	57.9	1163-1166 bp	1.0	81.1
ycf1_p4	CCAGTTCTCGTCACTTCCAG	TCAGACATAGGCCCCATTCA	55.0	50.0	57.9	58.1	1356-1359 bp	1.0	80.8
ycf1_p5	CCAAAGATTGCTCAGGCTGA	ATTGCTTCGCCAAAATTCCG	50.0	45.0	58.2	58.0	1283-1286 bp	0.666667	66.9