Hotspot

ndhG

SSC · 114469-115062 · 594 bp

Back to Cyrtomium

Pi 0.0109

MarkerSeek score 46.00

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 594
variable_sites: 15
pi: 0.0109
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	GAAAGGGCAGTAGCTAACCC	CTTGCGAAGTATGTGTCCGA	55.0	50.0	58.0	58.0	1407 bp	1.0	82.3
ndhG_p2	GAAAGGGCAGTAGCTAACCC	TTGCGAAGTATGTGTCCGAG	55.0	50.0	58.0	58.0	1406 bp	1.0	82.3
ndhG_p3	GAAAGGGCAGTAGCTAACCC	TGTCCGAGTATGTCCGATCA	55.0	50.0	58.0	58.0	1394 bp	1.0	82.1
ndhG_p4	GAAAGGGCAGTAGCTAACCC	TAGGTAGCAGGTTGGGTTCA	55.0	50.0	58.0	58.0	1631 bp	1.0	81.8
ndhG_p5	GAAAGGGCAGTAGCTAACCC	CCGTAGAAGCTGCGAGATAC	55.0	55.0	58.0	58.0	1560 bp	1.0	81.8