Hotspot

trnR-ACG-trnT-UGU

IRa · 148742-149484 · 743 bp

Back to Cyrtomium

Pi 0.00877

MarkerSeek score 58.00

Primer available yes

Label trnR-ACG-trnT-UGU

Feature Metadata

label_name: trnR-ACG-trnT-UGU
feature_type: igs
region: IRa
length_bp: 743
variable_sites: 14
pi: 0.00877
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnT-UGU_p1	CTCAGGGGATTAGAGCACGT	ATTGGTGAGCGTAACTCCAC	55.0	50.0	58.9	57.9	905-917 bp	1.0	74.5
trnR-ACG-trnT-UGU_p2	CTCAGGGGATTAGAGCACGT	AGTACGGAGGTTCGAATCCT	55.0	50.0	58.9	57.9	826-838 bp	1.0	74.3
trnR-ACG-trnT-UGU_p3	CTCAGGGGATTAGAGCACGT	GGATTGGTGAGCGTAACTCC	55.0	55.0	58.9	58.3	907-919 bp	1.0	72.4
trnR-ACG-trnT-UGU_p4	CTCAGGGGATTAGAGCACGT	GATTGGTGAGCGTAACTCCA	55.0	50.0	58.9	57.6	906-918 bp	1.0	72.2
trnR-ACG-trnT-UGU_p5	TCAGGGGATTAGAGCACGTG	ATTGGTGAGCGTAACTCCAC	55.0	50.0	59.2	57.9	904-916 bp	1.0	72.1