Hotspot

ndhE-ndhG

SSC · 114381-114468 · 88 bp

Back to Cyrtomium

Pi 0.0591

MarkerSeek score 68.20

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 88
variable_sites: 11
pi: 0.0591
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	CCAAAAATTAGTCCGTGTTCA	CCTTCATCATCTCAAAATAATTCTCCA	38.1	33.3	54.7	57.9	150 bp	1.0	45.9
ndhE-ndhG_p2	CCAAAAATTAGTCCGTGTTCAAAC	CCTTCATCATCTCAAAATAATTCTCCA	37.5	33.3	57.5	57.9	150 bp	1.0	45.9
ndhE-ndhG_p3	CCAAAAATTAGTCCGTGTTCAA	CCTTCATCATCTCAAAATAATTCTCCA	36.4	33.3	55.5	57.9	150 bp	1.0	45.9
ndhE-ndhG_p4	CCAAAAATTAGTCCGTGTTCA	CCTTCATCATCTCAAAATAATTCTCC	38.1	34.6	54.7	56.5	150 bp	1.0	45.9
ndhE-ndhG_p5	CCAAAAATTAGTCCGTGTTCAAA	CCTTCATCATCTCAAAATAATTCTCCA	34.8	33.3	56.2	57.9	150 bp	1.0	45.9