Hotspot

psaC-ndhE

SSC · 113733-114077 · 345 bp

Back to Cyrtomium

Pi 0.0213

MarkerSeek score 56.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 345
variable_sites: 16
pi: 0.0213
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGATTTTCACTGAATGTGCCA	GGGTTAGCTACTGCCCTTTC	38.1	55.0	56.1	58.0	432-458 bp	1.0	62.0
psaC-ndhE_p2	AGATTTTCACTGAATGTGCCA	GCCCTTTCTCTTCAGCGAAA	38.1	50.0	56.1	58.5	420-446 bp	1.0	58.7
psaC-ndhE_p3	AGATTTTCACTGAATGTGCCAT	GGGTTAGCTACTGCCCTTTC	36.4	55.0	56.6	58.0	432-458 bp	1.0	58.2
psaC-ndhE_p4	GATTTTCACTGAATGTGCCA	GGGTTAGCTACTGCCCTTTC	40.0	55.0	54.5	58.0	431-457 bp	1.0	57.4
psaC-ndhE_p5	AGATTTTCACTGAATGTGCCA	TACTGCCCTTTCTCTTCAGC	38.1	50.0	56.1	57.2	424-450 bp	1.0	56.3