Hotspot

rpl32-trnP-GGG

SSC · 109144-109785 · 642 bp

Back to Cyrtomium

Pi 0.0263

MarkerSeek score 54.80

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: SSC
length_bp: 642
variable_sites: 36
pi: 0.0263
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	GCAATAATTGACATCGGCGG	GAGGTCACAGGTTCGAATCC	50.0	55.0	58.0	58.0	1360-1362 bp	1.0	83.1
rpl32-trnP-GGG_p2	AGTGGAGCAGGGAAGATTCT	GAGGTCACAGGTTCGAATCC	50.0	55.0	58.0	58.0	1328-1329 bp	0.5	61.8
rpl32-trnP-GGG_p3	AGTGGAGCAGGGAAGATTCT	GGAGGTCACAGGTTCGAATC	50.0	55.0	58.0	58.0	1329-1330 bp	0.5	61.8
rpl32-trnP-GGG_p4	AGTGGAGCAGGGAAGATTCT	CAACGTCTATCTGCCCGAAA	50.0	50.0	58.0	58.0	1567-1570 bp	0.333333	54.6
rpl32-trnP-GGG_p5	GCAATAATTGACATCGGCGG	CAACGTCTATCTGCCCGAAA	50.0	50.0	58.0	58.0	1599-1602 bp	0.333333	54.6