Hotspot

trnT-UGU-trnR-ACG

IRb · 84058-84800 · 743 bp

Back to Cyrtomium

Pi 0.00877

MarkerSeek score 58.00

Primer available yes

Label trnT-UGU-trnR-ACG

Feature Metadata

label_name: trnT-UGU-trnR-ACG
feature_type: igs
region: IRb
length_bp: 743
variable_sites: 14
pi: 0.00877
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnR-ACG_p1	ATTGGTGAGCGTAACTCCAC	CTCAGGGGATTAGAGCACGT	50.0	55.0	57.9	58.9	905-917 bp	1.0	74.5
trnT-UGU-trnR-ACG_p2	AGTACGGAGGTTCGAATCCT	CTCAGGGGATTAGAGCACGT	50.0	55.0	57.9	58.9	826-838 bp	1.0	74.3
trnT-UGU-trnR-ACG_p3	GGATTGGTGAGCGTAACTCC	CTCAGGGGATTAGAGCACGT	55.0	55.0	58.3	58.9	907-919 bp	1.0	72.4
trnT-UGU-trnR-ACG_p4	GATTGGTGAGCGTAACTCCA	CTCAGGGGATTAGAGCACGT	50.0	55.0	57.6	58.9	906-918 bp	1.0	72.2
trnT-UGU-trnR-ACG_p5	ATTGGTGAGCGTAACTCCAC	TCAGGGGATTAGAGCACGTG	50.0	55.0	57.9	59.2	904-916 bp	1.0	72.1