Hotspot

rpl23-trnI-CAU

LSC · 81863-82034 · 172 bp

Back to Cyrtomium

Pi 0.0363
MarkerSeek score 65.70
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
LSC
length_bp
172
variable_sites
14
pi
0.0363
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 TGTTGCAACAAACGAATAGACT TAATTGGCGAATTCACGGGT 36.4 45.0 56.8 57.9 262-267 bp 1.0 59.9
rpl23-trnI-CAU_p2 TGTTGCAACAAACGAATAGACT ATTCACGGGTTCAACTCCTG 36.4 50.0 56.8 57.8 252-257 bp 1.0 59.6
rpl23-trnI-CAU_p3 TGTTGCAACAAACGAATAGACT CGGTTAAAGCACCCAACTCA 36.4 50.0 56.8 58.4 282-287 bp 1.0 57.2
rpl23-trnI-CAU_p4 TGTTGCAACAAACGAATAGACTT TAATTGGCGAATTCACGGGT 34.8 45.0 57.5 57.9 262-267 bp 1.0 57.2
rpl23-trnI-CAU_p5 TGTTGCAACAAACGAATAGACTT ATTCACGGGTTCAACTCCTG 34.8 50.0 57.5 57.8 252-257 bp 1.0 56.9