Hotspot

petA-psbJ

LSC · 59443-60148 · 706 bp

Back to Cyrtomium

Pi 0.0241

MarkerSeek score 57.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 35
pi: 0.0241
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTTGAAAAGGTGCAGTTGGC	GCCGGTACACTTGTGATAGG	45.0	55.0	57.7	58.1	819-832 bp	1.0	80.6
petA-psbJ_p2	TTTGAAAAGGTGCAGTTGGC	TGTGGTTGGTGGGTACTGTA	45.0	50.0	57.7	58.2	839-852 bp	1.0	79.5
petA-psbJ_p3	TTTGAAAAGGTGCAGTTGGC	CTGTAGCCGGTACACTTGTG	45.0	55.0	57.7	58.3	824-837 bp	1.0	78.8
petA-psbJ_p4	TTTGAAAAGGTGCAGTTGGC	TAGGCGTATTCCCTTACGGA	45.0	50.0	57.7	57.6	794-807 bp	1.0	78.3
petA-psbJ_p5	TTTGAAAAGGTGCAGTTGGC	GGTTCGTTAGGCGTATTCCC	45.0	55.0	57.7	58.4	801-814 bp	1.0	77.8