Hotspot

psbD-trnT-GGU

LSC · 33888-35236 · 1349 bp

Back to Cyrtomium

Pi 0.0127

MarkerSeek score 63.90

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1349
variable_sites: 40
pi: 0.0127
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	CCACGAAGACGAAACGATCT	TCTGAAGCCCCCTTAACTCA	50.0	50.0	58.0	58.0	1507-1521 bp	1.0	82.9
psbD-trnT-GGU_p2	CGAAACGATCTCTCCTGAGC	TCTGAAGCCCCCTTAACTCA	55.0	50.0	58.2	58.0	1498-1512 bp	1.0	81.8
psbD-trnT-GGU_p3	GAAACGATCTCTCCTGAGCC	TCTGAAGCCCCCTTAACTCA	55.0	50.0	57.8	58.0	1497-1511 bp	1.0	81.4
psbD-trnT-GGU_p4	GATCTCTCCTGAGCCAGTCA	TCTGAAGCCCCCTTAACTCA	55.0	50.0	58.2	58.0	1492-1506 bp	1.0	81.3
psbD-trnT-GGU_p5	TCTCTCCTGAGCCAGTCATC	TCTGAAGCCCCCTTAACTCA	55.0	50.0	58.2	58.0	1490-1504 bp	1.0	81.3