Hotspot

trnC-GCA-trnG-GCC

LSC · 29789-30192 · 404 bp

Back to Cyrtomium

Pi 0.0276

MarkerSeek score 56.80

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: LSC
length_bp: 404
variable_sites: 25
pi: 0.0276
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	CCTACGTCTTACCGCTTGAC	TCTTTGCCAAGGAGATGACG	55.0	50.0	58.1	57.9	475-478 bp	1.0	83.1
trnC-GCA-trnG-GCC_p2	GTCCTACGTCTTACCGCTTG	TCTTTGCCAAGGAGATGACG	55.0	50.0	58.1	57.9	477-480 bp	1.0	83.1
trnC-GCA-trnG-GCC_p3	AGTCCTACGTCTTACCGCTT	TCTTTGCCAAGGAGATGACG	50.0	50.0	58.2	57.9	478-481 bp	1.0	82.5
trnC-GCA-trnG-GCC_p4	TCCTACGTCTTACCGCTTGA	TCTTTGCCAAGGAGATGACG	50.0	50.0	58.2	57.9	476-479 bp	1.0	82.5
trnC-GCA-trnG-GCC_p5	TCGAACTGGGGGATTAAGGA	TCTTTGCCAAGGAGATGACG	50.0	50.0	57.7	57.9	503-506 bp	1.0	81.6