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psbM-petN

LSC · 27965-29171 · 1207 bp

Back to Cyrtomium

Pi 0.0161

MarkerSeek score 48.40

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 1207
variable_sites: 46
pi: 0.0161
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	GGCCGCGCAGAATAACTAG	ACTTGTAGTTCGGGGGAGAA	57.9	50.0	58.8	58.0	1257-1277 bp	1.0	68.6
psbM-petN_p2	GGCCGCGCAGAATAACTAG	CCCTCTCACTTGTAGTTCGG	57.9	55.0	58.8	57.3	1264-1284 bp	1.0	63.4
psbM-petN_p3	GGCCGCGCAGAATAACTAG	CCTCTCACTTGTAGTTCGGG	57.9	55.0	58.8	57.3	1263-1283 bp	1.0	63.4
psbM-petN_p4	GGCCGCGCAGAATAACTAG	CTCTCACTTGTAGTTCGGGG	57.9	55.0	58.8	57.3	1262-1282 bp	1.0	63.4
psbM-petN_p5	GGCCGCGCAGAATAACTAG	CTTGTAGTTCGGGGGAGAAG	57.9	55.0	58.8	57.3	1256-1276 bp	1.0	63.3