Hotspot

ndhA

SSC · 123749-125973 · 2225 bp

Back to Cyrtanthus

Pi 0.00764

MarkerSeek score 61.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2225
variable_sites: 17
pi: 0.00764
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATGAACCCGGTCACCATAGG	GTCCACCCGGTTTCATCAAT	55.0	50.0	59.2	58.2	2459-2460 bp	1.0	69.9
ndhA_p2	CCGGTCACCATAGGGAACAT	GTCCACCCGGTTTCATCAAT	55.0	50.0	59.2	58.2	2453-2454 bp	1.0	69.9
ndhA_p3	ATTTATGAACCCGGTCACCA	GTCCACCCGGTTTCATCAAT	45.0	50.0	56.8	58.2	2463-2464 bp	1.0	69.7
ndhA_p4	TTTATGAACCCGGTCACCAT	GTCCACCCGGTTTCATCAAT	45.0	50.0	56.8	58.2	2462-2463 bp	1.0	69.7
ndhA_p5	AATTTATGAACCCGGTCACCA	GTCCACCCGGTTTCATCAAT	42.9	50.0	57.6	58.2	2464-2465 bp	1.0	67.7